ProSci

GPRASP1 Antibody

Product Code:
 
PSI-7993
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Antibody Clone:
 
296080706
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
GPRASP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
1 / 3
Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.
2 / 3
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μg/ml.
3 / 3
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μg/ml.
❮❯

Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μg/ml.
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μg/ml.
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μg/ml.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-7993-0.02mg0.02mg£150.00
Quantity:
PSI-7993-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L
Application Note:
SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1,2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3,4).
Background References:
  • Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6.
  • Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77.
  • Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47.
  • Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.
Buffer:
SKI2W antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.

The immunogen is located within amino acids 60 - 110 of SKI2W.
NCBI Gene ID #:
6499
NCBI Official Name:
SKIV2L superkiller viralicidic activity 2-like (S. cerevisiae)
NCBI Official Symbol:
SKIV2L
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 113, 137 kDa

Observed: 135 kDa
Protein Accession #:
NP_008860
Protein GI Number:
67782311
Purification:
SKI2W antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q15477
User NOte:
Optimal dilutions for each application to be determined by the researcher.