Antibody Clonality:
Polyclonal
Target Species:- Bovine (Cattle)
- Human
- Porcine (pig)
- Rat
Applications:- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at 40 or in small aliquots for prolonged storage at ?200.
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:25 ? 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 ? 1:1000 for immunoblotting applications.
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiSerum is important in the differential diagnosis of bullous lesions in dermatopathology.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Cancer|Stem cell research
Each vial contains 250ul Rabbit polyclonal antiSerum including 0.09% sodium azide
Each vial contains 250ul Rabbit polyclonal antiSerum including 0.09% sodium azide
This polyclonal type IV collagen antibody is a Rabbit polyclonal antibody. The Rabbits were immunized with isolated Human type IV collagen. The immunogen has been isolated from Human placenta.
Polyclonal type IV collagen recognizes the extracellular ? basement membranes.
P08572, Q01955, P29400, P53420, P02462