ProSci

MCP-1/MCAF Antibody

Product Code:
 
PSI-XP-5218Bt
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fr
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To detect hMCP-1/MCAF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human MCP-1/MCAF (XP-5218) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hMCP-1/MCAF.
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To detect hMCP-1/MCAF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human MCP-1/MCAF (XP-5218) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hMCP-1/MCAF.

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CodeSizePrice
PSI-XP-5218Bt-0.05mg0.05mg£524.00
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This product comes from: United States.
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Further Information

Additional Names:
HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, C-C motif chemokine 2, HC11
Application Note:
ELISA:

Sandwich:
To detect hMCP-1/MCAF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MCP-1/MCAF (XP-5218) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-1/MCAF.

Western Blot:

To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-1(MCAF) (human Macrophage Chemoattractant Protein-1).
NCBI Gene ID #:
6347
NCBI Official Name:
chemokine (C-C motif) ligand 2
NCBI Official Symbol:
CCL2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13500
Protein GI Number:
126842
Purification:
Anti-hMCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P13500
User NOte:
Centrifuge vial prior to opening.