ProSci

I-309 Antibody

Product Code:
 
PSI-XP-5152
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
I-309 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas.  The recommended concentration is 0.25 ug/ml- 0.50 ug/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas.  The recommended concentration is 0.25 ug/ml- 0.50 ug/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas.  The recommended concentration is 0.25 ug/ml- 0.50 ug/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas.  The recommended concentration is 0.25 ug/ml- 0.50 ug/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary.

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PSI-XP-5152-0.1mg0.1mg£524.00
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Further Information

Additional Names:
P500, SISe, TCA3, I-309, SCYA1, C-C motif chemokine 1, Small-inducible cytokine A1,
Application Note:
ELISA:

To detect hI-309 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hI-309.

Sandwich: To detect hI-309 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human I-309 (XP-5152Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hI-309.

Western Blot:

To detect hI-309 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hI-309 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hI-309 (human I-309).
NCBI Gene ID #:
6346
NCBI Official Name:
chemokine (C-C motif) ligand 1
NCBI Official Symbol:
CCL1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P22362
Protein GI Number:
123950
Purification:
Anti-hI-309 specific antibody was purified by affinity chromatography employing immobilized hI-309 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P22362
User NOte:
Centrifuge vial prior to opening.