ProSci

FBXL12 Antibody

Product Code:
 
PSI-45-576
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-576 (4ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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45-576 (4ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

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PSI-45-576-0.1mg0.1mg£626.00
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Further Information

Additional Names:
FBXL12, likely ortholog of mouse f-box and leucine-rich repeat protein 12, FLJ20188, hypothetical protein FLJ20188, F-box and leucine-rich repeat protein 12, Fbl12
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Human Brain lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Lung shows staining of ciliated epithelial cells in the bronchioles. Recommended concentration, 4-6ug/ml.
Background References:
  • Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins. Curr Biol. 1999 Oct 21;9(20):1180-2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: RACPKESMDWWM
NCBI Gene ID #:
54850
NCBI Official Name:
F-box and leucine-rich repeat protein 12
NCBI Official Symbol:
FBXL12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060173.1
Protein GI Number:
8923179
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9NXK8