ProSci

CXCR3 Antibody

Product Code:
 
PSI-45-439
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-439 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue
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45-439 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue

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PSI-45-439-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Background References:
  • Martini G, Zulian F, Calabrese F, Bortoli M, Facco M, Cabrelle A, Valente M, Zacchello F, Agostini C. CXCR3/CXCL10 expression in the synovium of children with juvenile idiopathic arthritis. Arthritis Res Ther. 2005;7(2):R241-9. Epub 2005 Jan 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RRDSSWSETSEA
NCBI Gene ID #:
2833
NCBI Official Name:
chemokine (C-X-C motif) receptor 3
NCBI Official Symbol:
CXCR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001495.1, NP_001136269.1
Protein GI Number:
4504099
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P49682