ProSci

BIN1 Antibody

Product Code:
 
PSI-45-332
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-332 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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45-332 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

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PSI-45-332-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
BIN1, bridging integrator 1, AMPH2, AMPHL, DKFZp547F068, MGC10367, SH3P9, amphiphysin II, amphiphysin-like, box dependant MYC interacting protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Experiments gave bands at approx 70kDa and 60kDa in Human Skeletal Muscle lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Chang et al, Cancer Res. 2007 Jan 1;67(1):100-7.; PMID: 17210688). This protein has a calculated MW of 64.7kDa according to NP_647593.1. Recommended concentration: 1-3ug/ml.
Background References:
  • Ghaneie A, Zemba-Palko V, Itoh H, Itoh K, Sakamuro D, Nakamura S, Soler AP, Prendergast GC. Bin1 attenuation in breast cancer is correlated to nodal metastasis and reduced survival. Cancer Biol Ther. 2007 Feb;6(2):192-4. Epub 2007 Feb 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SDNAPAKGNKSPS
NCBI Gene ID #:
274
NCBI Official Name:
bridging integrator 1
NCBI Official Symbol:
BIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004296.1, NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_647600.1, NP_647601.1
Protein GI Number:
4757748
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise all ten reported isoforms (NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_004296.1, NP_647600.1, NP_647601.1).
Swissprot #:
O00499