Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NSUN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
NSUN2, FLJ20303, MISU, SAKI, TRM4, MRT5
NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).
- Huttenhofer, A., (2007) Mol. Biol. Cell 18 (3), 1107-1117.
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
batch dependent
Unconjugated
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.
54888
NOP2/Sun RNA methyltransferase family, member 2
NSUN2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
86 kDa
NP_060225
39995082
Antibody is purified by peptide affinity chromatography method.
Q08J23
Optimal dilutions for each application to be determined by the researcher.