ProSci

AMPKa1 Antibody

Product Code:
 
PSI-79-185
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser487) Antibody.
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser487).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1(Phospho-Ser487)Antibody #11174.
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Western blot analysis of lysed extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser487) Antibody.
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser487).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1(Phospho-Ser487)Antibody #11174.

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PSI-79-185-0.1mg0.1mg£488.00
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Further Information

Additional Names:
AMPK, AMPKa1, FLK1, VEGFR2, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Background References:
  • Kim JE, et al. (2005) J Proteome Res. 4 (4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278 (31): 28434-28442.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AMPKa1 (Phospho-Ser487) antibody was raised in was raised against a peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKα1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5562
NCBI Official Name:
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol:
PRKAA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
NP_006242.5
Protein GI Number:
94557301
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of AMPKα1 only when phosphorylated at serine 487.
Swissprot #:
Q13131/P54646