ProSci

CD19 Monoclonal Antibody

Product Code:
 
PSI-32-129
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2E2B6B10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
CD19 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.
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Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.

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PSI-32-129-0.1mg0.1mg£404.00
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Further Information

Additional Names:
B-lymphocyte surface antigen B4B4, CVID3
Application Note:
Western Blot:Dilution 1:2000 - 5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
The CD19 antigen (95 kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
Background References:
  • Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187.
  • Rie, M.A. de, Leukaemia Research, 1988. 12: 135.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.
NCBI Gene ID #:
930
NCBI Official Name:
CD19 molecule
NCBI Official Symbol:
CD19
NCBI Organism:
Homo sapiens
Protein Accession #:
P15391
Protein GI Number:
160332376
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Immunology
Swissprot #:
P15391
User NOte:
Optimal dilutions for each application to be determined by the researcher.