ProSci

PAK1 Antibody

Product Code:
 
PSI-46-127
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-127 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-127 (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-127-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) , MGC130000, MGC130001, PAKalpha , p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex and Hippocampus) lysates (calculated MW of 60.6kDa according to NP_002567.3). Recommended concentration: 0.5-1.5ug/ml.
Background References:
  • Zhao L, Ma QL, Calon F, Harris-White ME, Yang F, Lim GP, Morihara T, Ubeda OJ, Ambegaokar S, Hansen JE, Weisbart RH, Teter B, Frautschy SA, Cole GM. Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease. Nat Neurosci. 2006 Feb;9(2):234-42. Epub 2006 Jan 15.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-NTEKQKKKPKMSDE
NCBI Gene ID #:
5058
NCBI Official Name:
p21 protein (Cdc42/Rac)-activated kinase 1
NCBI Official Symbol:
PAK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002567.3
Protein GI Number:
42794769
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
No cross-reactivity exptected with PAK3
Swissprot #:
Q13153