Further Information
NUP62, p62, MGC841, FLJ20822, DKFZp547L134, nucleoporin 62kDa, nuclear pore glycoprotein p62, FLJ43869, IBSN, SNDI
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.3kDa according to NP_036478.2, NP_057637.2, NP_714940.1 and NP_714941.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- Carmo-Fonseca M, Kern H, Hurt EC. Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur J Cell Biol. 1991 Jun;55(1):17-30.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
The immunogen for this antibody is: C-RKEQERSFRITFD
23636
nucleoporin 62kDa
NUP62
Homo sapiens
Liquid
NP_036478.2, NP_057637.2, NP_714940.1, NP_714941.1
24497603
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
All four variants represent identical protein.
P37198