ProSci

VPS29 Antibody

Product Code:
 
PSI-46-582
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-582 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
❮❯

46-582 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-46-582-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
VPS29, vacuolar protein sorting 29 (yeast) , DC15, DC7, DKFZp564F0223, FLJ20492, PEP11 , retromer protein, vacuolar protein sorting 29, vacuolar protein sorting 29 (yeast homolog), vacuolar sorting protein VPS29/PEP11, x 007 protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5kDa according to NP_057310.1 and 20.9kDa according to NP_476528.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands. Recommended concentration, 4-8ug/ml.
Background References:
  • Nakada-Tsukui K, Saito-Nakano Y, Ali V, Nozaki T. A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica. Mol Biol Cell. 2005 Nov;16(11):5294-303. Epub 2005 Aug 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: CTGNLCTKESYDYLK
NCBI Gene ID #:
51699
NCBI Official Name:
vacuolar protein sorting 29 homolog (S. cerevisiae)
NCBI Official Symbol:
VPS29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057310.1, NP_476528.1
Protein GI Number:
7706441
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).
Swissprot #:
Q9UBQ0