Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
UGT8, UDP glycosyltransferase 8, CGT, UGT4, 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, UDP-galactose ceramide galactosyltransferase, UDP-galactose-ceramide galactosyltransferase, ceramide UDP-galactosyltransferase, cerebroside synthase, uridine diphosphate glycosyltransferase 8
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kDa is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kDa according to NP_003351.2. Recommended concentration, 1-3ug/ml.
Preliminary experiments gave an approx 50kDa band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kDa according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Cow
The immunogen for this antibody is: C-NGKYKRNGHIK
7368
UDP glycosyltransferase 8
UGT8
Homo sapiens
Liquid
NP_003351.2
40254471
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Reported variants represent identical protein: NP_001121646.1, NP_003351.2.
Q16880