Further Information
SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) , HGNC:11179, ALS, ALS1, IPOA, SOD , Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154), superoxide dismutase 1, soluble, superoxide dismutase, cystolic
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03?g/ml. Primary incubation 1 hour at room temperature. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SOD1 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
- Ghadge GD, Wang L, Sharma K, Monti AL, Bindokas V, Stevens FJ, Roos RP. Truncated wild-type SOD1 and FALS-linked mutant SOD1 cause neural cell death in the chick embryo spinal cord. Neurobiol Dis. 2006 Jan;21(1):194-205. Epub 2005 Aug 9
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Dog
The immunogen for this antibody is: C-SRKHGGPKDEERH
6647
superoxide dismutase 1, soluble
SOD1
Homo sapiens
Liquid
NP_000445.1
4507149
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
P00441