ProSci

SIRT3 Antibody

Product Code:
 
PSI-46-372
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-372 (0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-372 (0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-372-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
SIRT3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae), HGNC:14931, SIR2L3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3, sirtuin 3, sirtuin type 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Human Liver lysates (calculated MW of 28.6kDa according to NP_001017524.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QRETGKLDGPDK
NCBI Gene ID #:
23410
NCBI Official Name:
sirtuin 3
NCBI Official Symbol:
SIRT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036371.1, NP_001017524.1
Protein GI Number:
6912660
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
Swissprot #:
Q9NTG7