ProSci

SIPA1 Antibody

Product Code:
 
PSI-46-371
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-371 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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46-371 staining (1ug/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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PSI-46-371-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1, signal-induced proliferation-associated 1, MGC102688, signal-induced proliferation-associated gene 1, signal-induced proliferation-associated protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31;272(44):28081-8; PMID: 9346962). Recommended concentration: 1-3ug/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.
Background References:
  • Hattori M, Tsukamoto N, Nur-e-Kamal MS, Rubinfeld B, Iwai K, Kubota H, Maruta H, Minato N. Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression. Mol Cell Biol. 1995 Jan;15(1):552-60.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ASKQLGSPTADLA
NCBI Gene ID #:
6494
NCBI Official Name:
signal-induced proliferation-associated 1
NCBI Official Symbol:
SIPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_694985.28, NP_006738.3
Protein GI Number:
24497629
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Both variants represent identical protein (NP_694985.28 and NP_006738.3).
Swissprot #:
Q96FS4