ProSci

PJA1 Antibody

Product Code:
 
PSI-43-019
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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CodeSizePrice
PSI-43-019-0.1mg0.1mg£505.00
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Further Information

Additional Names:
PJA1, praja ring finger 1, PRAJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, OTTHUMP00000023457, OTTHUMP00000023458, OTTHUMP00000158771, RING finger protein 70, praja 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Brain and Mouse Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.4kDa according to NP_071763.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse
Immunogen:
The immunogen for this antibody is: C-HSTSRWRDTANDNE
NCBI Gene ID #:
64219
NCBI Official Name:
praja ring finger 1, E3 ubiquitin protein ligase
NCBI Official Symbol:
PJA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 30 kDa
Protein Accession #:
NP_660095.1, NP_001027568.1, NP_071763.2
Protein GI Number:
41281725
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_660095.1; NP_001027568.1; NP_071763.2).
Swissprot #:
Q8NG27