ProSci

PIKE Antibody

Product Code:
 
PSI-46-997
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-997 (0.3ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-997 (0.3ug/ml) staining of Human Amygdala lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-46-997-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
CENTG1, centaurin, gamma 1, AGAP2, FLJ16430, GGAP2, KIAA0167, PIKE, Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2, GTP-binding and GTPase activating protein 2, centaurin gamma1, phosphoinositide 3-kinase enhancer
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in Human Brain (Amygdala and Cerebral Cortex) lysates (calculated MW of 90.5kDa according to NP_055585.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid and Kidney. Recommended concentration: 3.75ug/ml.
Background References:
  • Liu Z, Jang SW, Liu X, Cheng D, Peng J, Yepes M, Li XJ, Matthews S, Watts C, Asano M, Hara-Nishimura I, Luo HR, Ye K. Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase. Mol. Cell 2008 Mar 29 (6): 665-78.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QASLDSIREAVINSQ
NCBI Gene ID #:
116986
NCBI Official Name:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
NCBI Official Symbol:
AGAP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055585.1
Protein GI Number:
7661962
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody is expected to recognize isoform PIKE-S (NP_055585.1).
Swissprot #:
Q99490