ProSci

PCK2 Antibody

Product Code:
 
PSI-46-146
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-146 (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-146 (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-146-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in Human Liver lysates (calculated MW of 70.7kDa according to NP_004554.2). Recommended concentration: 2-4ug/ml.
Background References:
  • Duplus E, Benelli C, Reis AF, Fouque F, Velho G, Forest C. Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes. Biochimie. 2003 Dec;85(12):1257-64.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: KPWKPGDKEPCAH
NCBI Gene ID #:
5106
NCBI Official Name:
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
NCBI Official Symbol:
PCK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004554.2
Protein GI Number:
66346721
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
Swissprot #:
Q16822