LY6E, lymphocyte antigen 6 complex, locus E, RIG-E, RIGE, SCA-2, SCA2, TSA-1, retinoic acid induced gene E
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot:Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry:Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep;34(9):831-5. PMID: 23830620.
Immunofluorescence: Strong expression of the protein seen in the membranes of NIH3T3 and L929 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.