ProSci

COL4A6 Antibody

Product Code:
 
PSI-42-181
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
 

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CodeSizePrice
PSI-42-181-0.1mg0.1mg£565.00
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This product comes from: United States.
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Further Information

Additional Names:
COL4A6, collagen, type IV, alpha 6, MGC88184, OTTHUMP00000023834, OTTHUMP00000023835, collagen IV, alpha-6 polypeptide, collagen alpha 6 type IV, collagen of basement membrane, alpha-6, dJ889N15.4 (Collagen Alpha 6(IV)), type IV alpha 6 collagen
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Zhang X, Zhou J, Reeders ST, Tryggvason K Structure of the human type IV collagen COL4A6 gene, which is mutated in Alport syndrome-associated leiomyomatosis Genomics. 1996 May 33 (3): 473-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-PSPEFETETLHNKES
NCBI Gene ID #:
1288
NCBI Official Name:
collagen, type IV, alpha 6
NCBI Official Symbol:
COL4A6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001838.2, NP_378667.1
Protein GI Number:
148536823
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_001838.2; NP_378667.1).
Swissprot #:
Q14031