APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot:Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.
Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6ug/ml.
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.