ProSci

TSFM Antibody

Product Code:
 
PSI-25-011
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TSFM antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 1
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
❮❯

Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

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PSI-25-011-100ul100ul£662.00
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Further Information

Additional Names:
TSFM, COXPD3, EF-TS, EF-Tsmt, EFTS, EFTSMT
Application Note:
TSFM antibody can be used for detection of TSFM by ELISA at 1:312500. TSFM antibody can be used for detection of TSFM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The TSFM protein is a mitochondrial translation elongation factor. Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes.Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).
Background References:
  • Smeitink, J.A., (2006) Am. J. Hum. Genet. 79 (5), 869-877.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TSFM.
NCBI Gene ID #:
10102
NCBI Official Name:
Ts translation elongation factor, mitochondrial
NCBI Official Symbol:
TSFM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
NP_005717
Protein GI Number:
171846268
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
P43897
User NOte:
Optimal dilutions for each application to be determined by the researcher.