ProSci

LIGHT Antibody

Product Code:
 
PSI-XP-5215Bt
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
LIGHT antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
1 / 3
To detect hLIGHT by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hLIGHT.
2 / 3
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 3
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
❮❯

To detect hLIGHT by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hLIGHT.
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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CodeSizePrice
PSI-XP-5215Bt-0.05mg0.05mg£524.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
LTg, TR2, CD258, HVEML, LIGHT, UNQ391/PRO726, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L
Application Note:
ELISA:

Sandwich:
To detect hLIGHT by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human LIGHT (XP-5215) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Western Blot:

To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT (human LIGHT).
NCBI Gene ID #:
8740
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 14
NCBI Official Symbol:
TNFSF14
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O43557
Protein GI Number:
313104028
Purification:
Anti-hLIGHT specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Infectious Disease,Apoptosis,Chemokines & Cytokines,Herpes,Antibody Pairs
Swissprot #:
O43557
User NOte:
Centrifuge vial prior to opening.