ProSci

IL-10 Monoclonal Antibody

Product Code:
 
PSI-38-301
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with ProSci's biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.
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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with ProSci's biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

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CodeSizePrice
PSI-38-301-0.5mg0.5mg£385.00
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Further Information

Additional Names:
Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
Application Note:
ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci?s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
E.coli derived Recombinant Murine IL-10
NCBI Gene ID #:
3586
NCBI Official Name:
interleukin 10
NCBI Official Symbol:
IL10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_000563.1
Protein GI Number:
10835141
Purification:
This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
Research Area:
Infectious Disease,Neuroscience,Cancer,Stem Cell,Chemokines & Cytokines, Functional Assays
Swissprot #:
P22301
User NOte:
Centrifuge vial prior to opening.