ProSci

MCP-2 Antibody

Product Code:
 
PSI-XP-5220
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
MCP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
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To detect hMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MCP-2 (XP-5220Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.
2 / 7
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 7
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 7
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
5 / 7
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
6 / 7
This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
7 / 7
This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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To detect hMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MCP-2 (XP-5220Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal gastric mucosa. The recommended concentration is 0.75mg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

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PSI-XP-5220-0.1mg0.1mg£524.00
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This product comes from: United States.
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Further Information

Additional Names:
HC14, MCP2, MCP-2, SCYA8, SCYA10, C-C motif chemokine 8, HC14
Application Note:
ELISA:

To detect hMCP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCP-2.

Sandwich:

To detect hMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MCP-2 (XP-5220Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.

Western Blot:
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-2 (human Macrophage/Monocyte chemotactic protein-2).
NCBI Gene ID #:
6355
NCBI Official Name:
chemokine (C-C motif) ligand 8
NCBI Official Symbol:
CCL8
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P80075
Protein GI Number:
2506280
Purification:
Anti-hMCP-2 specific antibody was purified by affinity chromatography employing immobilized hMCP-2 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P80075
User NOte:
Centrifuge vial prior to opening.