ProSci

MCP-3 Antibody

Product Code:
 
PSI-XP-5223
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
MCP-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
1 / 4
To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
2 / 4
To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
3 / 4
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 4
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
❮❯

To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
To detect mMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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CodeSizePrice
PSI-XP-5223-0.1mg0.1mg£524.00
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Further Information

Additional Names:
fic, marc, mcp3, MCP-3, Scya7, Fic, Mcp3, C-C motif chemokine 7, Monocyte chemoattractant protein 3
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine MCP-3 (100.0 ng/mL), a concentration of 4.0 - 6.0 μg/mL of this antibody is required..

ELISA:

To detect mMCP-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-3.

Sandwich:

To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-3 (XP-5223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.

Western Blot:
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine Macrophage/Monocyte chemotactic protein-3).
NCBI Gene ID #:
20306
NCBI Official Name:
chemokine (C-C motif) ligand 7
NCBI Official Symbol:
Ccl7
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q03366
Protein GI Number:
417192
Purification:
Anti-mMCP-3 specific antibody was purified by affinity chromatography employing immobilized mMCP-3 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q03366
User NOte:
Centrifuge vial prior to opening.