ProSci

PPARD Antibody

Product Code:
 
PSI-45-118
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-118 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-118 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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CodeSizePrice
PSI-45-118-0.1mg0.1mg£626.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferative activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Background References:
  • Park BH, Vogelstein B, Kinzler KW. Genetic disruption of PPARdelta decreases the tumorigenicity of human colon cancer cells. Proc Natl Acad Sci USA. 2001 Feb 27;98(5):2598-603.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: CHPLLQEIYKDMY
NCBI Gene ID #:
5467
NCBI Official Name:
peroxisome proliferator-activated receptor delta
NCBI Official Symbol:
PPARD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006229.1
Protein GI Number:
5453940
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Swissprot #:
Q03181