ProSci

NDRG1 Antibody

Product Code:
 
PSI-45-938
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-938 (0.1ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-938 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-938 (0.1ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-938 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-938-0.1mg0.1mg£626.00
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Further Information

Additional Names:
NDRG1, N-myc downstream regulated gene 1, CAP43,, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein regulated by oxygen-1, reducing agents and tunicamycin-responsive protein, tunicamycin-responsive protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2ug/ml. Paraffin embedded Human Prostate and Colon. Recommended concentration: 2.5ug/ml.
Background References:
  • Zhou D, Salnikow K, Costa M. Cap43, a novel gene specifically induced by Ni2+ compounds. Cancer Res. 1998 May 15;58(10):2182-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: GNSAGPKSMEVSC
NCBI Gene ID #:
10397
NCBI Official Name:
N-myc downstream regulated 1
NCBI Official Symbol:
NDRG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006087.2
Protein GI Number:
37655183
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
The variants (NP_006087.2; NP_001128714.1) encode the same protein
Swissprot #:
Q92597