Further Information
NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
Peptide ELISA: antibody detection limit dilution 1:20000.
Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1). The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature.
Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.
Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002 Apr 1;363(Pt 1):95-103
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Cow
The immunogen for this antibody is: C-SESTKRKLASAV
653361
neutrophil cytosolic factor 1
NCF1
Homo sapiens
Liquid
NP_000256.4
115298672
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeostasis
.
P14598