Further Information
IGF1, insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot:Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature.
Negative Control: HepG2 cell lysate. Preliminary testing was unsuccessful on?Mouse and Rat?for this particular batch.
Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
The immunogen for this antibody is: C-RSVRAQRHTD
3479
insulin-like growth factor 1 (somatomedin C)
IGF1
Homo sapiens
Liquid
NP_001104753.1, NP_001104754.1, NP_001104755.1, NP_000609.1
163659899
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
P05019