Further Information
TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5kDa according to Human NP_003290.1 and Mouse NP_035761.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3?g/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5?g/ml. Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10?g/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, Evans J, Herbert M, Hyslop L, Ahmad S, Murdoch A, Strachan T. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells. 2004;22(5):790-7.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow, Zebrafish
The immunogen for this antibody is: C-KEGVKFDESEKTKE
7184
heat shock protein 90kDa beta (Grp94), member 1
HSP90B1
Homo sapiens
Liquid
NP_003290.1
4507677
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neuroscience
.
P14625