ProSci

bNGF Monoclonal Antibody

Product Code:
 
PSI-38-239
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2
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This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary.
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This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary.

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CodeSizePrice
PSI-38-239-0.5mg0.5mg£488.00
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Further Information

Additional Names:
Nerve growth factor, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 , Beta-nerve growth factorBeta-NGF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:

To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
NCBI Gene ID #:
4803
NCBI Official Name:
nerve growth factor (beta polypeptide)
NCBI Official Symbol:
NGF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01138
Protein GI Number:
90110037
Purification:
This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
Research Area:
Neuroscience, Functional Assays
Swissprot #:
P01138
User NOte:
Centrifuge vial prior to opening.