ProSci

Integrin beta3 Antibody

Product Code:
 
PSI-79-490
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HUVEC cells using Integrin β3 (Phospho-Tyr785).
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Western blot analysis of lysed extracts from HUVEC cells using Integrin β3 (Phospho-Tyr785).

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PSI-79-490-0.1mg0.1mg£488.00
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Further Information

Additional Names:
GT, CD61, GP3A, BDPLT2, GPIIIa, BDPLT16, RAF, CD61 antigen, ITB3, Platelet membrane glycoprotein IIIa
Application Note:
Western Blot: 1:500~1:1000
Background:
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Background References:
  • Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397 (Pt 3): 437?447Courter DL, et al. (2005) J Biol Chem Apr 01; 280 (13): 12145-12151Chandhoke SK, et al. (2004) J Cell Sci Mar 15; 117 (Pt 8): 1431-1441
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Integrin beta3 (Phospho-Tyr785) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 785 (I-T-Y (p) -R-G) derived from Human Integrin β3.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3690
NCBI Official Name:
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
NCBI Official Symbol:
ITGB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
NP_000203.2
Protein GI Number:
47078292
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Integrin β3 only when phosphorylated at tyrosine 785.
Swissprot #:
P05106