ProSci

CDK4 Antibody

Product Code:
 
PSI-79-654
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store antibody at -20˚C for up to one year.
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Western blot analysis of lysed extracts from HUVEC cells using CDK4 Antibody.
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Western blot analysis of lysed extracts from HUVEC cells using CDK4 Antibody.

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PSI-79-654-0.1mg0.1mg£481.00
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Further Information

Additional Names:
CMM3, PSK-J3, PRL3, CMM3
Application Note:
Western Blot: 1:500~1:1000
Background:
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
Background References:
  • Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681. Sherr, C. J. (1996) Science 274, 1672-1677. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925. Gabrielli, B. G. et al. (1999) J Biol Chem 274, 13961-9.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CDK4 antibody was raised against a peptide sequence around aa.295~299 (L-H-K-D-E) derived from Human CDK4. .
NCBI Gene ID #:
1019
NCBI Official Name:
cyclin-dependent kinase 4
NCBI Official Symbol:
CDK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
NP_000066.1
Protein GI Number:
4502735
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous levels of total CDK4 protein.
Swissprot #:
P11802