ProSci

Nogo 66 Receptor Antibody

Product Code:
 
PSI-45-975
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-975 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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45-975 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-975-0.1mg0.1mg£626.00
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Further Information

Additional Names:
RTN4R, Nogo-66 receptor, reticulon 4 receptor, HGNC:1860, NGR, NOGOR, UNQ330/PRO526, nogo receptor, reticulon 4 receptor precursor, OTTHUMP00000197866
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3ug/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1ug/ml.
Background References:
  • Shao Z, Browning JL, Lee X, Scott ML, Shulga-Morskaya S, Allaire N, Thill G, Levesque M, Sah D, McCoy JM, Murray B, Jung V, Pepinsky RB, Mi S. TAJ/TROY, an orphan TNF receptor family member, binds Nogo-66 receptor 1 and regulates axonal regeneration. Neuron. 2005 Feb 3;45(3):353-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Dog
Immunogen:
The immunogen for this antibody is: C-LQALPDDTFRDLGN
NCBI Gene ID #:
65078
NCBI Official Name:
reticulon 4 receptor
NCBI Official Symbol:
RTN4R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_075380.1
Protein GI Number:
13194201
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9BZR6