ProSci

EndoPDI Antibody

Product Code:
 
PSI-45-536
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-536 staining (0.1ug/ml) of HEK293 (A), A549 (B), HeLa (C) and (0.01ug/ml) of HepG2 (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-536 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain
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45-536 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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45-536 staining (0.1ug/ml) of HEK293 (A), A549 (B), HeLa (C) and (0.01ug/ml) of HepG2 (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-536 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain
45-536 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

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PSI-45-536-0.1mg0.1mg£626.00
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Further Information

Additional Names:
TXNDC5, EndoPDI, thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide, thioredoxin domain containing 5 (endoplasmic reticulum) isomerase, Hcc-2, PDIA15, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain containing 5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in lysates of cell lines HEK293, A549, HeLa and HepG2 (calculated MW of 47.6kDa according to NP_110437.2). The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Sullivan DC, Huminiecki L, Moore JW, Boyle JJ, Poulsom R, Creamer D, Barker J, Bicknell R. EndoPDI, a novel protein-disulfide isomerase-like protein that is preferentially expressed in endothelial cells acts as a stress survival factor. J Biol Chem. 2003 Nov 21;278(47):47079-88. Epub 2003 Sep 08.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SLHRFVLSQAKDEL
NCBI Gene ID #:
81567
NCBI Official Name:
thioredoxin domain containing 5 (endoplasmic reticulum)
NCBI Official Symbol:
TXNDC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_110437.2, NP_001139021.1
Protein GI Number:
42794771
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.
Swissprot #:
Q8NBS9