Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
TRIM63, MURF1, MURF-1, RNF28, ring finger protein 28, IRF, SMRZ, MURF2, FLJ32380, iris ring finger protein, muscle specific ring finger protein 2, striated muscle RING zinc finger protein, tripartite motif-containing 63, muscle specific ring finger protein 1
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Recommended concentration: 1.25ug/ml.
- Dai KS, Liew CC. A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J Biol Chem. 2001 Jun 29;276(26):23992-9.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: DYKSSLIQDGNPM-C
84676
tripartite motif containing 63, E3 ubiquitin protein ligase
TRIM63
Homo sapiens
Liquid
NP_115977.2
19924163
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q969Q1