Product Code:
PSI-XP-5199Bt
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-8 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8
ELISA:
Sandwich:
To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-8 (XP-5199) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8.
Western Blot:
To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
batch dependent
Biotin
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8).
3576
interleukin 8
IL8
Homo sapiens
Lyophilized
P10145
124359
Anti-hIL-8 specific antibody was purified by affinity chromatography and then biotinylated.
Immunology,Chemokines & Cytokines,Antibody Pairs
P10145
Centrifuge vial prior to opening.