ProSci

IL-3B Antibody

Product Code:
 
PSI-XP-5187
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
IL-3B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
1 / 3
To detect Rat IL-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IL-3-beta (XP-5187Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3-beta.
2 / 3
To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 3
To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
❮❯

To detect Rat IL-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IL-3-beta (XP-5187Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3-beta.
To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IL-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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CodeSizePrice
PSI-XP-5187-0.1mg0.1mg£524.00
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This product comes from: United States.
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Further Information

Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3β (150 ng/mL), a concentration of 0.68 - 1.03 μg/mL of this antibody is required.

ELISA:

To detect Rat IL-3β by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β..

Sandwich:

To detect Rat IL-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-3β (XP-5187Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β.

Western Blot:

To detect Rat IL-3β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3β (rat IL-3 beta).
NCBI Gene ID #:
24495
NCBI Official Name:
interleukin 3
NCBI Official Symbol:
Il3
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P97688
Protein GI Number:
81870605
Purification:
Anti-rat IL-3β specific antibody was purified by affinity chromatography employing immobilized hIL-3β matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P97688
User NOte:
Centrifuge vial prior to opening.