ProSci

CDCP1 Antibody

Product Code:
 
PSI-45-031
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-031 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-031 (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-031-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CDCP1, CUB domain containing protein 1 , HGNC:24357, CD318, SIMA135, TRASK , CUB domain-containing protein 1, transmembrane and associated with src kinases
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5ug/ml.
Background References:
  • Hooper JD, Zijlstra A, Aimes RT, Liang H, Claassen GF, Tarin D, Testa JE, Quigley JP Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003 Mar 27;22(12):1783-94.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-PLLNTQEPMEPAE
NCBI Gene ID #:
64866
NCBI Official Name:
CUB domain containing protein 1
NCBI Official Symbol:
CDCP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_073753.3
Protein GI Number:
30410805
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Swissprot #:
Q9H5V8