Further Information
PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory subunit-8, ARD-1, RNase E, protein phosphatase 1 regulatory inhibitor subunit 8, protein phosphatase 1 regulatory subunit 8
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung for this particular batch.Immunohistochemistry:In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Beullens M, Bollen M. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem. 2002 May 31;277(22):19855-60. Epub 2002 Mar 21.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: C-EAWPGKKPTPSLLI
5511
protein phosphatase 1, regulatory subunit 8
PPP1R8
Homo sapiens
Liquid
NP_002704.1, NP_054829.2, NP_612568.1
4506015
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise all three human isoforms of this protein.
Q12972