ProSci

EPHB2 Antibody

Product Code:
 
PSI-45-544
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-544 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-544 (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-544-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
EPHB2, DRT, EPH receptor B2, Cek5, Qek5, Sek3, ETECK, Prkm5, EPHT3, ERK, Hek5, MGC87492, Tyro5, developmentally-regulated eph-related tyrosine kinase, elk-related tyrosine kinase, eph tyrosine kinase 3, ephrin receptor EphB2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in Human Lung and Placenta lysates (calculated MW of 110kDa according to NP_059145.2 and NP_004433.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Litterst et al, J Biol Chem. 2007 Apr 10; PMID: 17428795). Recommended concentration: 1-3ug/ml.
Background References:
  • Cramer KS, Cerretti DP, Siddiqui SA. EphB2 regulates axonal growth at the midline in the developing auditory brainstem. Dev Biol. 2006 Apr 13;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QKDRNHRPKFGQ
NCBI Gene ID #:
2048
NCBI Official Name:
EPH receptor B2
NCBI Official Symbol:
EPHB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004433.2, NP_059145.2
Protein GI Number:
21396504
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms ( NP_059145.2 and NP_004433.2).
Swissprot #:
P29323