ProSci

UBE2D1 Antibody

Product Code:
 
PSI-29-819
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2D1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
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Antibody used in WB on recombinant protein at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
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Antibody used in WB on recombinant protein at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).

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PSI-29-819-100ul100ul£609.00
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Further Information

Additional Names:
UBE2D1, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Application Note:
UBE2D1 antibody can be used for detection of UBE2D1 by ELISA at 1:62500. UBE2D1 antibody can be used for detection of UBE2D1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2D1.
NCBI Gene ID #:
7321
NCBI Official Name:
ubiquitin-conjugating enzyme E2D 1
NCBI Official Symbol:
UBE2D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa, 42 kDa
Protein Accession #:
NP_003329
Protein GI Number:
4507773
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Membrane ,Cancer ,Signal Transduction
Swissprot #:
P51668
User NOte:
Optimal dilutions for each application to be determined by the researcher.