ProSci

PPP2R3A Antibody

Product Code:
 
PSI-46-701
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-701 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-701 (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-701-0.1mg0.1mg£626.00
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Further Information

Additional Names:
PPP2R3A, protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha , PPP2R3, PR130, PR72, PP2A, subunit B, B''-PR72/PR130, PP2A, subunit B, B72/B130 isoforms, PP2A, subunit B, R3 isoform, Serine/threonine protein phosphatase 2A, 72/130 kDa regul
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5ug/ml.
Background References:
  • Zhang S, Cagatay T, Amanai M, Zhang M, Kline J, Castrillon DH, Ashfaq R, Oz OK, Wharton KA Jr. Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12):4454-64. Epub 2007 Apr 16.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QKDVENDGPEPSD
NCBI Gene ID #:
5523
NCBI Official Name:
protein phosphatase 2, regulatory subunit B'', alpha
NCBI Official Symbol:
PPP2R3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002709.2, NP_871626.1
Protein GI Number:
19923229
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
Swissprot #:
Q06190