ProSci

VEGF Antibody

Product Code:
 
PSI-XP-5740-M
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Clonality:
 
Monoclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
 
VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&#
1 / 1
To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with ProSci's biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 ug/ml.
❮❯

To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 100 pg/ml of recombinant human VEGF165 when used with ProSci's biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 ug/ml.

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CodeSizePrice
PSI-XP-5740-M-0.5mg0.5mg£488.00
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Further Information

Additional Names:
Vascular Endothelial Growth Factor, VEGF, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μg/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL.

Western Blot:

To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.
NCBI Gene ID #:
7422
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
VEGFA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P15692
Protein GI Number:
17380528
Purification:
This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
Research Area:
Signal Transduction,Growth Factor, Functional Assays
Swissprot #:
P15692
User NOte:
Centrifuge vial prior to opening.