Further Information
FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot:Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3ug/ml. Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Coon H, Matsunami N, Stevens J, Miller J, Pingree C, Camp NJ, Thomas A, Krasny L, Lainhart J, Leppert MF, McMahon W. Evidence for linkage on chromosome 3q25-27 in a large autism extended pedigree. Hum Hered. 2005;60(4):220-6. Epub 2006 Jan 2.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
The immunogen for this antibody is: C-RIEGDNENKLPRED
8087
fragile X mental retardation, autosomal homolog 1
FXR1
Homo sapiens
Liquid
NP_005078.2, NP_001013456.1, NP_001013457.1
61835148
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neuroscience
This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
P51114