ProSci

UBR2 Antibody

Product Code:
 
PSI-42-209
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
42-209 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-209 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-42-209-0.1mg0.1mg£626.00
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Further Information

Additional Names:
UBR2, ubiquitin protein ligase E3 component n-recognin 2, C6orf133, DKFZp686C08114, KIAA0349, MGC71112, RP3-392M17.3, bA49A4.1, dJ242G1.1, dJ392M17.3, OTTHUMP00000016403, OTTHUMP00000016405, OTTHUMP00000039767, OTTHUMP00000039768, ubiquitin ligase E3 alpha-II
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 150kDa in Rat Testis lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199kDa according to Rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Brain (Cortex). Recommended concentration: 5ug/ml.
Background References:
  • Zenker M, Mayerle J, Lerch MM, Tagariello A, Zerres K, Durie PR, Beier M, H?lskamp G, Guzman C, Rehder H, Beemer FA, Hamel B, Vanlieferinghen P, Gershoni-Baruch R, Vieira MW, Dumic M, Auslender R, Gil-da-Silva-Lopes VL, Steinlicht S, Rauh M, Shalev SA, Thiel C, Ekici AB, Winterpacht A, Kwon YT, Varshavsky A, Reis A. Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome). Nature genetics 2005 Dec 37 (12): 1345-50.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: SCPKSGGDKSRAPT
NCBI Gene ID #:
23304
NCBI Official Name:
ubiquitin protein ligase E3 component n-recognin 2
NCBI Official Symbol:
UBR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056070.1
Protein GI Number:
27597061
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
Swissprot #:
Q8IWV8