ProSci

DDAH1 Antibody

Product Code:
 
PSI-45-468
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-468 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-468 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-468-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in Human Kidney, Human Brain (Cerebellum) and Pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul;19(9):1175-7; PMID: 15827267). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Kimoto M, Miyatake S, Sasagawa T, Yamashita H, Okita M, Oka T, Ogawa T, Tsuji H. Purification, cDNA cloning and expression of human NG, NG-dimethylarginine dimethylaminohydrolase. Eur J Biochem. 1998 Dec 1;258(2):863-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: TCCSVLINKKVDS
NCBI Gene ID #:
23576
NCBI Official Name:
dimethylarginine dimethylaminohydrolase 1
NCBI Official Symbol:
DDAH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036269.1, NP_001127917.1
Protein GI Number:
6912328
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Swissprot #:
O94760